Universal Sequencing TELL-Seq Library
Zofotokozera
- Dzina lazogulitsa: TELL-SeqTM Library Sequencing Kit
- Wopanga: Universal Sequencing Technology Corporation
- Kugwirizana: Makina onse otsatizana a Illumina kupatula iSeq 100
- Kugwiritsa Ntchito Kofuna: Kugwiritsa Ntchito Kafukufuku Pokha, osati njira zowunikira
- Mbiri Yobwereza: Mtundu 7.0, Ogasiti 2024
Malangizo Ogwiritsira Ntchito Zogulitsa:
Mawu Oyamba
Laibulale ya TELL-Seq TM imafuna zoyambira zotsatizana za machitidwe aliwonse a Illumina ndipo ili ndi 18-base index 1 sequence yomwe imagwiritsidwa ntchito ngati ma barcode a molecular for linked reads, yomwe iyenera kutsatiridwa kwathunthu.
Zamkatimu Zamkati
| Dzina lachigawo | Mtundu wa Cap | Kukhazikika | Voliyumu (µL) |
|---|---|---|---|
| Werengani 1 Koyamba | CAP Black | 100M | 50 |
| Werengani 2 Koyamba | CAP White | 100M | 50 |
| Mndandanda wa 1 Woyamba | CAP Red | 100M | 50 |
| Mndandanda wa 2 Woyamba | CAP Yellow | 100M | 50 |
TELL-SeqTM Laibulale ya Kapangidwe ndi Sequencing Scheme
- Mlozera 1 (I7 index) uli ndi 18-base TELL Bead sequencers zomwe ziyenera kutsatiridwa kwathunthu.
- Mlozera 2 (I5 index) uli ndi sample index primer sequences zomwe zimagwiritsidwa ntchito mu library ampkumangirira.
- Kuphatikizika kophatikizana kophatikizana kumakondedwa ndi kuchuluka kwautali wofunikira wa 2 × 96 komanso kutalika kofunikira kwa 2 × 150.
- Ma library amatha kuphatikizidwa pamodzi kuti atsatidwe pamene zoyambira zingapo zamitundu yosiyanasiyana zimagwiritsidwa ntchito mulaibulale ampsitepe ya liification.
Zoyambira Zotsata Mwamakonda
Zoyambira zotsatirira mwamakonda zimafunikira kuti zitsatire malaibulale a TELL-SeqTM ndipo zimaperekedwa mu TELL-SeqTM Illumina Sequencing Primer Kit.
Custom Index 2 Primer
Choyambirira cha Index 2 chimafunika pamene malaibulale ambiri a TELL-SeqTM okhala ndi ma multiplex oyambira amasonkhanitsidwa kuti atsatidwe komanso pamene sequencer imafuna i5 index sequencing primer. Pakadali pano, kwa MiSeq kokha, chizolowezi cha Index 2 Primer sichifunikira.
Mafunso Ofunsidwa Kawirikawiri (FAQ)
- Q: Kodi zida za TELL-SeqTM zitha kugwiritsidwa ntchito pochiza matenda?
A: Ayi, zida za TELL-Seq TM ndizogwiritsa Ntchito Kafukufuku Pokha ndipo siziyenera kugwiritsidwa ntchito pochiza matenda. - Q: Kodi kutalika kocheperako komanso kopitilira muyeso kowerengera kumafunikira pamayendedwe apawiri?
A: Chofunikira chaching'ono chowerengera ndi 2 × 96 ndipo kutalika kofunikira kuti muwerenge ndi 2 × 150 pakutsatana komaliza.
TELL-Seq™ Library Sequencing User Guide
Kwa machitidwe onse a Illumina@ kupatula iSeq 100
Kugwiritsa Ntchito Kafukufuku Pokha. Osagwiritsidwa ntchito pozindikira matenda.
Chikalata # 100018 v7.0
Ogasiti 2024
Chikalatachi ndi cha mwini wake wa Universal Sequencing Technology Corporation ndipo cholinga chake ndi kugwiritsidwa ntchito ndi kasitomala wake pokhudzana ndi kugwiritsa ntchito zomwe zafotokozedwa pano osati zolinga zina.
Malangizo omwe ali m'chikalatachi akuyenera kutsatiridwa ndendende ndi anthu ophunzitsidwa bwino kuti awonetsetse kuti zida za TELL-Seq™ zikugwiritsidwa ntchito moyenera komanso motetezeka.
UNIVERSAL SEQUENCING TECHNOLOGY SIKUGANIZA NTCHITO ILIYONSE ZOMWE ZIKUCHITIKA MAKAGWIRITSA NTCHITO MALOWA KA TELL-SEQ™ KIT.
©2022 Universal Sequencing Technology Corporation. Maumwini onse ndi otetezedwa.
TELL-Seq™ ndi chizindikiro cha Universal Sequencing Technology Corporation. Mayina ena onse, ma logo ndi zilembo zina ndi katundu wa eni ake.
Mbiri Yobwereza
| Chikalata # | Baibulo | DCR Reference ndi Ndemanga |
| C01K002 Rev. A | Januware 2020 | Kutulutsidwa Koyamba |
| C01K002 Rev. B | Marichi 2020 | Onjezani Zowonjezera |
| 100018-USG | 3.0 | Onjezani kalozera wa NovaSeq v1.5 reagent |
| 100018-USG | 4.0 | Onjezani malangizo atsatanetsatane a NovaSeq yaposachedwa
reagents |
| 100018-USG | 5.0 | Onjezani kalozera wa NextSeq 1000/2000 system |
| 100018-USG | 6.0 | Onjezani zambiri za 96 Multiplex primers (C-series). |
| 100018-USG | 7.0 | Onjezani zoyambira 384 Multiplex (C,D,E,F-mndandanda)
zambiri. Sinthani zambiri za Index 2 Primer |
Mawu Oyamba
Protocol iyi ikufotokoza momwe mungayendetsere malaibulale aliwonse omwe ali ndi indexed-pained-TELL-Seq™ pamayendedwe otsatizana a Illumina®.
Laibulale ya TELL-Seq™ † imafuna zoyambira zotsatirira pamakina aliwonse a Illumina ndipo ili ndi 18-base index 1 sequence yomwe imagwiritsidwa ntchito ngati ma barcode a molekyulu yowerengera zolumikizidwa, zomwe ziyenera kutsatiridwa kwathunthu.
Zamkatimu Zamkati
TELL-Seq™ Illumina® Sequencing Primer Kit (PN 100004)
TELL-Seq™ Illumina® Sequencing Primer Kit, HT (PN 100013)
TELL-Seq™ Laibulale ya Kapangidwe ndi Sequencing Scheme
Mlozera 1 (ie, I7 index) uli ndi magawo 18 a TELL Bead, omwe AYENERA kutsatidwa kwathunthu. Index 2 (ie, I5 index) ili ndi 8-base (T-series) kapena 10-base (C-series)ample index primer sequences zomwe zimagwiritsidwa ntchito mu library ampkufotokozera. Kutsatira malekezero awiri ndikwabwino. Chofunikira chaching'ono chowerengera ndi 2 × 96; Chofunikira pakuwerenga kwambiri ndi 2 × 150.
Example ya Illumina® Sequencing % Base by Cycle Chart
Illumina® Sequencing Guideline
- Chepetsani laibulale ya TELL-Seq™ molingana ndi Illumina® yotsatizana ndi nsanja komanso kuchuluka kwake.
- Malaibulale akhoza kuphatikizidwa pamodzi kuti atsatidwe pamene machulukitsidwe osiyanasiyana akugwiritsidwa ntchito mulaibulale ampsitepe ya liification.
- Zoyambira zotsatirira makonda zimafunikira kuti zitsatire malaibulale a TELL-Seq™ ndikuperekedwa mu TELL-Seq™ Illumina Sequencing Primer Kit.
- Zoyambira zotsatizanazi zitha kukwezedwa m'zitsime zomwe zafotokozedwa kuti ziziyambira. Kapenanso, amathanso kukwezedwa m'zitsime zofananira za Illumina® pomwe laibulale yoyang'anira ya Illumina® PhiX ikutsatiridwa motsatizana.
- Custom Index 2 primer imafunika kokha pamene malaibulale angapo a TELL-Seq™ okhala ndi ma multiplex oyambilira aphatikizidwa kuti atsatidwe komanso pamene chotsatira chikufuna choyambira cha i5 index. Pakadali pano kwa MiSeq kokha, chizolowezi cha Index 2 Primer sichifunikira. Komanso pamakina osagwiritsidwa ntchito ngati HiSeq 2000/2500 ndi NovaSeq v1 reagents, chizolowezi cha Index 2 choyambirira sichifunikira.
- Nambala yocheperako yotsatizana imatha kuchitidwa pogwiritsa ntchito kuchuluka kwa zoyambira zomwe zaperekedwa zimasiyanasiyana malinga ndi dongosolo lotsatirira.
| Sequencing System | Kodi Custom Index 2 Primer ikufunika? |
| NovaSeq X/X Plus | Inde |
| NovaSeq 6000 | Inde |
| HiSeq 3000/4000 | Inde |
| NextSeq | Inde |
| MiSeq | Ayi |
| MiniSeq | Inde |
Exampndi Sample Mapepala a MiSeq System
Pansipa pali exampndi sample sheet la 2 × 146 PE kuthamanga ndi 18-cycle index 1 (ie, I7 index) ndi 8-cycle kapena 10-base (C-series) index 2 (ie, I5 index) yotsatizana pogwiritsa ntchito zoyambira zotsatizana za Werengani 1 , Index 1 ndi Werengani 2 zomwe zidzalowetsedwa mu zitsime zoyambira zotsatizana. Chifukwa MiSeq imagwiritsa ntchito P5 primer pa cell cell cell monga choyambira chotsatira cha Index 2 chimawerengedwa, TELL-Seq™ custom Index 2 primer sichifunikira pa MiSeq system. Kuchulukitsa kwa malaibulale aliwonse ophatikizidwa kudzakhazikitsidwa pa sample index (ie, index 2). Idzakonzedwa ndi TELL-read analysis pipeline padera pakamaliza kutsatizana.
Illumina® Sequencing Read Length Recommendation
- Kutsatana kophatikizana kophatikizana kumalimbikitsidwa.
- TELL-Seq™ library Index 1 ndi 18-base, Index 2 ndi 8-base (T-series) kapena 10-base (C-series). Pali 26-base (T-series) kapena 28-base (C-series) pazolozera zonse ziwiri poyerekeza ndi 16-base yokhazikika ya Illumina dual index.
- Zowonjezera 10-mkombero wofunikira pakutsata index ya laibulale ya TELL-Seq™ ikuyenera kuchotsedwa pa kuwerenga 1 ndikuwerenga mizere iwiri yotsatizana. Popeza Illumina sequencing reagent imatsimikizira 2 zowonjezera, 2-cycle kuti muwerenge 4 ndi 1-cycle kuti muwerenge 4 iyenera kuchotsedwa motsatira.
- Kutalika kotsatizana kovomerezeka ndi 2 × 96 PE mpaka 2 × 146 PE pawiri index index; 2 × 100 PE mpaka 2 × 150 PE kwa mphindi imodziample kuthamanga popanda kufunika kwa Index 2 kuwerenga.
Kutsata Kuganizira Mozama
- Kuya kokwanira kotsatizana kumafunika kuti mumve zambiri za TELL Bead. Momwe TELL Beads amagwiritsidwa ntchito mulaibulale amplification kuti mupange laibulale ya TELL-Seq™, mawerengedwe otsatizana kwambiri amafunikira kuti mupeze kuzama komwe mukufuna. Komabe, TELL Beads ochepa omwe amagwiritsidwa ntchito ku library amplification, kutsika kwa laibulale kudzakhala kovuta, zomwe zingayambitse kuchulukitsa kwa chiwerengero chowerengera. Mulingo wapakati pa TELL Beads wogwiritsidwa ntchito ndi TELL-Seq™ laibulale yovuta yofunikira ingadalire kukula kwa genome ndi kagwiritsidwe ntchito.
- Pogwiritsa ntchito msonkhano wa de novo, osachepera 60x ma genome a sample akulimbikitsidwa ambiri. Komabe, kusanja kocheperako kungakhalenso kokwanira kutengera kuchuluka kwa TELL Beads zomwe zimagwiritsidwa ntchito ku library amplification ndi zovuta za library ya TELL-Seq™.
- For human phasing, at least 500 million cluster reads per sample yomwe ili ~ 40x kuya ndiyofunikira.
- Pakutsatizana komwe mukufuna, kuwunikira kochepera 100x kumalimbikitsidwa.
Library Multiplex Primer Index Sequences (ie, Index 2 Read): T-series (8-base)
| Library Multiplex
Primer (T-series) |
Kwa Sampndi Mapepala MiSeq | Kwa Sampndi Mapepala NovaSeq,
NovaSeq X, NextSeq, MiniSeq |
| T501 | Mtengo wa TGAACCTT | AAGGTTCA |
| T502 | Mtengo wa TGCTAAGT | ACTTAGCA |
| T503 | Mtengo wa TGTCTCT | AGAGACA |
| T504 | Mtengo wa TAAGACAC | Chithunzi cha GTGTCTTA |
| T505 | Mtengo wa CTAATCGA | Mtengo wa TCGATTAG |
| T506 | CTAGAACA | Mtengo wa TGTTCTAG |
| T507 | Mtengo wa TAGTTCC | GGAACTTA |
| T508 | TAGMtengo wa ACCTA | TAGZithunzi za GTCTA |
| T509 | CATCCGAA | Mtengo wa TTCGGATG |
| T510 | Chithunzi cha TTATGAGT | ACTCATAA |
| T511 | AGGCGC | GCCTCT |
| T512 | TAGCCGCG | CGCGGCTA |
| T513 | ACGAATAA | Chithunzi cha TTTTTGT |
| T514 | Mtengo wa TTCGTAGG | CCTACGAA |
| T515 | Mtengo wa GACTGCT | Mtengo wa AGCAGATC |
| T516 | CGCTCCGC | Mtengo wa GCGGAGCG |
| T517 | AGGCTATA | TATAGMtengo CCT |
| T518 | Mtengo wa GCTCTAT | ATAGMtengo wa AGGC |
| T519 | AGGATAGG | Chithunzi cha CCTATCCT |
| T520 | Mtengo wa TCAGAGCC | GGCTCTGA |
| T521 | Chithunzi cha CTTCGCCT | Mtengo wa AGGCGAAG |
| T522 | TAAGATTA | Chithunzi cha TAATCTTA |
| T523 | Mtengo wa AGTAAGTA | TACTTACT |
| T524 | Zotsatira GACTTCCT | Mtengo wa AGGAAGTC |
Library Multiplex Primer Index Sequences (ie Index 2 Read): C,D,E,F-series (10-base)
| Library Multiplex Primer (C-series) | Kwa Sampndi Mapepala MiSeq | Kwa Sampndi Mapepala NovaSeq, NovaSeq X, NextSeq, MiniSeq |
| C501 | Chithunzi cha ACGTACGTAC | GTACGTACGT |
| C502 | Chithunzi cha CATGCATGCA | TGCATGCATG |
| C503 | GTACGTACGT | Chithunzi cha ACGTACGTAC |
| C504 | TGCATGCATG | Chithunzi cha CATGCATGCA |
| C505 | Mtengo wa ATGCTGATCA | Mtengo wa TGATCCAT |
| C506 | Chithunzi cha CACAGCTGTG | Chithunzi cha CACAGCTGTG |
| C507 | GCTGATCAGC | GCTGATCAGC |
| C508 | Mtengo wa TGATCCAT | Mtengo wa ATGCTGATCA |
| C509 | ATTCAATACT | Mtengo wa AGTATTGAAT |
| C510 | CTAGCGCTAG | CTAGCGCTAG |
| C511 | GCTAGTAGTA | Mtengo wa TACTACTAGC |
| C512 | Mtengo wa TCCAATCAAG | Chithunzi cha CTTGATTGGA |
| C513 | Chithunzi cha AATATTGCTG | Chithunzi cha CAGCAATATT |
| C514 | CGTCGTTACG | Mtengo wa CGTAACGACG |
| C515 | Mtengo wa GATTGATTCC | Mtengo wa GGAATCAATC |
| C516 | Mtengo wa TCTACAATG | Zotsatira CATTGTTAGA |
| C517 | GAATTTGTCA | Mtengo wa TGACAATCT |
| C518 | Chithunzi cha CTCAGCAATT | Zotsatira zamagulu AATTGCTGAG |
| C519 | GGTCCTTGTC | Mtengo wa GACAAGGACC |
| C520 | Mtengo wa AGGCCTGACA | Chithunzi cha TGTCAGGCCT |
| C521 | Mtengo wa CTCCTAGTGG | CCACTAGGAG |
| C522 | Mtengo wa GGTTACAGCT | Mtengo wa AGCTGTAACC |
| C523 | Chithunzi cha CTGATTGGCG | CGCCAATCAG |
| C524 | Chithunzi cha ATTGGTTAGA | Mtengo wa TCTAACCAAT |
| C525 | Chithunzi cha CCATCAACT | Mtengo wa AGTTGAATGG |
| C526 | Zotsatira CAGTATTGAC | Mtengo wa GTCAATACTG |
| C527 | GAGTCCTCAA | Mtengo wa TTGAGGACTC |
| C528 | AGCTACTACT | AGTAGTAGCT |
| C529 | TAGCTAGCGC | GCGCTAGMtengo CTA |
| C530 | Mtengo wa GATGCACAC | Chithunzi cha GTGTTGCATC |
| C531 | CCTCAGTACA | Mtengo wa TGTACTGAGG |
| C532 | CGGTATTCA | Mtengo wa TGAATTACCG |
| C533 | CGCAATGGCT | AGCATTGCG |
| C534 | GTACGTTGAA | Mtengo wa TTCAACGTAC |
| C535 | TTGATCAGTA | TACTGATCAA |
| C536 | GGCCTAACAA | Chithunzi cha TTGTTAGMtengo wa GCC |
| C537 | GTTGTTGGAA | Chithunzi cha TTCCAACAAC |
| C538 | Mtengo wa TACGTTGGAC | GTCCAACGTA |
| C539 | ACACCATGCA | Chithunzi cha TGCATGGTGT |
| C540 | GCAATAGTAC | Chithunzi cha GATATTGC |
| C541 | ACGCAGCCAG | Chithunzi cha CTGCTGCGT |
| C542 | Mtengo wa CGAGTTGACG | Chithunzi cha CGTCAACTCG |
| C543 | Mtengo CGTGCTGAA | TTCAGCCACG |
| C544 | Mtengo wa TCCAAGGAC | GTCCTTGAGA |
| C545 | CCTAGGCACT | Mtengo wa AGTGCCTAGG |
| C546 | Mtengo wa CTGCGGTAAT | Zotsatira ATTACCGCAG |
| C547 | GGCACTACCA | TGGTAGMtengo wa TGCC |
| C548 | Mtengo wa GCTCAATCAA | Mtengo wa TTGATTGAGC |
| C549 | Mtengo wa AGGCACACAC | Chithunzi cha GTGTGCCT |
| C550 | CCTGGCAAGA | Mtengo wa TCTTGCCAGG |
| C551 | Mtengo wa TATTGGTAG | Mtengo wa CTACCAATTA |
| C552 | GCCAACAAGT | Chithunzi cha ACTGTTGGC |
| C553 | Chithunzi cha ATGGCTTATA | TATAAGCCAT |
| C554 | Chithunzi cha GCATGGCCTT | AAGGCCATGC |
| C555 | Malingaliro a kampani ACAATACTGG | CCAGTATTGT |
| C556 | GGATTGGACT | Mtengo wa AGTCCAATCC |
| C557 | ACTGTACTAT | ATAGMtengo wa TACAGT |
| C558 | Chithunzi cha CAGCTGTGAG | Mtengo wa CTCAAGCTG |
| C559 | Chithunzi cha CTTGAGGACC | GGTCTCCAAG |
| C560 | Mtengo wa GGTACAATAG | Mtengo wa CTTTGTACC |
| C561 | Mtengo wa CTGACTACTA | TAGTAGMtengo wa TCAG |
| C562 | Chithunzi cha TCAACCATGG | CCATGGTTGA |
| C563 | ATTATAACCG | Mtengo wa CGGTTATAAT |
| C564 | ACTAGMtengo wa TCCTT | Mtengo wa AAGGACTAGT |
| C565 | Mtengo wa ACTTGGACGT | Mtengo wa ACTCCAAGT |
| C566 | Zithunzi za ATGGTTAGGA | Mtengo wa TCCTAACCAT |
| C567 | Chithunzi cha ATGTACCAA | Chithunzi cha TTGTACCAT |
| C568 | Malingaliro a kampani GAATTGACTC | GAGTCAATTC |
| C569 | AGCAACCAGG | Chithunzi cha CCTGGTTGCT |
| C570 | Mtengo wa TACTGTGCTG | CAGCACAGTA |
| C571 | Chithunzi cha CAACAACGTC | Chithunzi cha GACGTTGTTG |
| C572 | CAGTAGMtengo wa CGCT | Mtengo wa AGCGCTACTG |
| C573 | Zotsatira ATTACCAATC | GATTGGTAAT |
| C574 | Mtengo wa TAAGGACCGC | Chithunzi cha GCGTCCTTA |
| C575 | Mtengo wa ACACGTACCG | Chithunzi cha CGGTACGTGT |
| C576 | Chithunzi cha CAACGTTGTT | AACAACGTTG |
| C577 | Chithunzi cha ATTGCTGA | Mtengo wa TCAGCACAAT |
| C578 | Mtengo wa GTACCCAACAG | Chithunzi cha CTGTTGGTAC |
| C579 | TTGTCAAGGA | Mtengo wa TCCTTGACAA |
| C580 | Chithunzi cha CTTGTACGTA | Malingaliro a kampani TACGTACAAG |
| C581 | TGCCTTGTAA | Mtengo wa TTACAGCA |
| C582 | TAGTAGMtengo wa CTTA | Mtengo wa TAAGCTACTA |
| C583 | GACCCAATG | Chithunzi cha CATTGCGGTC |
| C584 | Mtengo wa CTACTAGMtengo CTT | AAGCTAGTAG |
| C585 | Mtengo wa AGCACGTT | Chithunzi cha AACGTGCT |
| C586 | TGTTATAAGC | GCTTATAACA |
| C587 | GTTGCCAAGT | Mtengo wa ACTTGCAAC |
| C588 | Chithunzi cha CTGCAACCG | Mtengo wa CGGTTGCCAG |
| C589 | TTAGGCCTTA | TAGGCCTAA |
| C590 | Mtengo wa CGCAGCACAG | Chithunzi cha CTGTGCTGCG |
| C591 | CTAGGCCAA | Chithunzi cha TTGTGCCTAG |
| C592 | Mtengo wa TGTTGTACAG | Chithunzi cha CTGTACACA |
| C593 | Chithunzi cha CTAACGTGGC | Mtengo wa GCCACGTTAG |
| C594 | GCGTACTGGT | ACCAGTACGC |
| C595 | Mtengo wa GGCCTGAATT | AATTCAGGCC |
| C596 | Chithunzi cha CATGCTCGAG | Chithunzi cha CTCGAGCATG |
| Library Multiplex Primer (D-series) | Kwa Sampndi Mapepala MiSeq | Kwa Sampndi Mapepala NovaSeq, NovaSeq X, NextSeq, MiniSeq |
| D501 | AGCACTGTAA | Mtengo wa TTACAGTGCT |
| D502 | Mtengo wa CCGAAGTACT | AGTACTTCGG |
| D503 | Chithunzi cha GTTCAAGTA | Zotsatira TACTTGTAAC |
| D504 | Chithunzi cha TGATTGCGTG | Chithunzi cha CACGCAATCA |
| D505 | Chithunzi cha ATCTTAGGCA | Mtengo wa TGCCTAAGAT |
| D506 | Chithunzi cha CACGCAAGTT | Chithunzi cha AACTTGCGTG |
| D507 | Mtengo wa GAGCTTAGGA | Mtengo wa TCCTAAGCTC |
| D508 | Mtengo wa TATTGCTCGA | TCGAGCAATA |
| D509 | Malingaliro a kampani AACATCTGAG | Chithunzi cha CTCAGATGTT |
| D510 | Chithunzi cha CAACTCAGCA | Mtengo wa TGCTGAGTTG |
| D511 | GAACTATATG | CATATAGMtengo wa TTC |
| D512 | TAATAGMtengo wa TCTA | TAGACTATTA |
| D513 | Chithunzi cha AAGATTGACG | CGTCAATCTT |
| D514 | Mtengo CACGTAGCCG | Chithunzi cha CGGCTCGTG |
| D515 | GACTCGATCA | Mtengo wa TGATCGAGTC |
| D516 | Chithunzi cha TAATCCTCGC | Mtengo wa GCGAGGATTA |
| D517 | AAGCTCCTGG | CCAGGAGCTT |
| D518 | Chithunzi cha CAGATTGTTG | Malingaliro a kampani CACACAACTG |
| D519 | Mtengo wa GATGCTATTC | GAATAGZotsatira CATC |
| D520 | Chithunzi cha TAATCCTCGC | Mtengo wa GCGAGGATTA |
| D521 | Chithunzi cha AAGTATACCT | Mtengo wa AGGATACTT |
| D522 | CAGTAGCCAA | Mtengo wa TTGGCTACTG |
| D523 | Zotsatira GCAAGGACTC | GAGTCCTTGC |
| D524 | Mtengo wa TACTGTA | Mtengo wa TACAGTAGTA |
| D525 | Mtengo wa AATCCTAGCT | AGCTAGGATT |
| D526 | Zotsatira CATTGCAAG | Chithunzi cha CTTGCCAATG |
| D527 | GCCTGAGAAT | Mtengo wa ATTCAGGC |
| D528 | TACTAGCAAG | Mtengo CTTGCTAGTA |
| D529 | Mtengo wa ACAGCTAGTC | GACTAGMtengo wa CTGT |
| D530 | CCAACCAGTA | Mtengo wa TACTGGTTGG |
| D531 | GCTCAATT | Chithunzi cha AATTGAGCGC |
| D532 | TAGMtengo wa CGCGGAC | GTCCGCGCTA |
| D533 | Mtengo wa ACCTATAGTT | AACTATAGGT |
| D534 | Chithunzi cha CCACGTCAGG | Chithunzi cha CCTGACGTGG |
| D535 | Mtengo wa GCTACCAAT | Mtengo wa ATTGCGTAGC |
| D536 | TAGMtengo wa TTGCTGC | GCAGCAACTA |
| D537 | Mtengo wa ACGGTACAAC | Chithunzi cha GTTGTACCGT |
| D538 | CCGAACTTCG | Chithunzi cha CGAAGTTCGG |
| D539 | Mtengo wa GCTCCTAATT | AATTAGGAGC |
| D540 | Mtengo wa TTCCTCGAT | Chithunzi cha ATCGAGGATA |
| D541 | ACTCGATCT | AGAATCGAGT |
| D542 | Chithunzi cha CCTGGTAATT | Chithunzi cha AATTACCAGG |
| D543 | Mtengo wa GCTTAGMtengo wa TTGG | Chithunzi cha CCAACTAAGC |
| D544 | TCAGCAAGGA | Mtengo wa TCCTTGCTGA |
| D545 | Mtengo wa ACTGGTAGCA | Chithunzi cha TGCTACCAGT |
| D546 | Mtengo wa CGATCAACCT | AGGTTGATCG |
| D547 | Mtengo wa GGAAGTAGCA | Mtengo wa TGCTACTTCC |
| D548 | Chithunzi cha TCAGCTGCGG | CCGCAGCTGA |
| D549 | Mtengo wa ACTGGTAGCA | Chithunzi cha TGCTACCAGT |
| D550 | Mtengo wa CGATCAACCT | AGGTTGATCG |
| D551 | GGCAAGATGA | Mtengo wa TCATCTTGCC |
| D552 | TCCGCCAATG | Chithunzi cha CATTGCGGA |
| D553 | AGAACTTAT | ATAAGATTCT |
| D554 | CGCTAGCAAC | Zithunzi za GTTGCTAGCG |
| D555 | GGCCTTATAT | Mtengo wa ATATAAGGCC |
| D556 | Mtengo wa TCGAATACTA | TAGTATTCGA |
| D557 | AGCAATGGTA | Mtengo wa TACCATGCT |
| D558 | CGGTCTGATC | GATCGACCG |
| D559 | GGTCGCCCAT | Chithunzi cha ATGCGGACC |
| D560 | Mtengo wa TCGAATACTA | TAGTATTCGA |
| D561 | AGCACTGTAA | Mtengo wa TTACAGTGCT |
| D562 | Chithunzi cha CGTACTACCG | CGGTAGMtengo wa TACG |
| D563 | GGTTCATCGG | Mtengo wa CCGATGAACC |
| D564 | Mtengo wa TCGGAGCTGC | GCAGCTCCGA |
| D565 | Mtengo wa AGGACGTTAG | Chithunzi cha CTAACGTCCT |
| D566 | Mtengo wa CGTTGACAAT | Mtengo wa ATGTCAACG |
| D567 | Mtengo wa GTACTGACAC | Mtengo wa GGTCAGTAC |
| D568 | TCTAAGATAG | Chithunzi cha CTATCTTAGA |
| D569 | Mtengo wa AGCTCAAC | Zotsatira GTTGAGCACT |
| D570 | Mtengo wa CTAAGAACCT | Mtengo wa AGGTCTTAG |
| D571 | Chithunzi cha GATATTAC | Chithunzi cha GTAATATAC |
| D572 | Chithunzi cha TGATTGCGTG | Chithunzi cha CACGCAATCA |
| D573 | Mtengo wa ATTTGACG | CGTCGAATAT |
| D574 | Mtengo wa CTATAACGAC | Zithunzi za GTCGTTATAG |
| D575 | Zithunzi za GTCGTTAGGT | ACCTACGAC |
| D576 | Mtengo wa TGCCACTGAT | Chithunzi cha ATCAGTGCA |
| D577 | Zotsatira ATCGGCTAAG | CTTAGChithunzi cha CCGAT |
| D578 | Mtengo wa CTCAAGTTGC | Zotsatira GCAACTTGAG |
| D579 | GTGTGTTCGA | Mtengo wa TCGAACACAC |
| D580 | Mtengo wa TGCTATAGTC | GACTATAGCA |
| D581 | Mtengo wa ATGAGCTATT | AATAGZotsatira CTCAT |
| D582 | Mtengo wa CTCACAACGA | Mtengo wa TCGTTGTGAG |
| D583 | Chithunzi cha TGCTCGATTG | CAATCGAGCA |
| D584 | Zotsatira ATGTTGACT | Mtengo wa AGTCCAACAT |
| D585 | Chithunzi cha CTGATGTTAG | Mtengo wa CTAACATCAG |
| D586 | Mtengo wa TGGTAACAG | Chithunzi cha CTGGTTACCA |
| D587 | Zotsatira ATTCGAAGTA | Zotsatira TACTTCGAAT |
| D588 | CTTAGMtengo wa magawo CTCCT | Mtengo wa AGGAGCTAAG |
| D589 | TGTAATCGAT | Mtengo wa ATCGATTACA |
| D590 | Malingaliro a kampani CTTGGACTTC | GAAGTCCAAG |
| D591 | TGTACTTGAA | Mtengo wa TTCAAGTACA |
| D592 | TTAAGCTCAG | CTGAGCTTAA |
| D593 | Mtengo wa TTACAACCAG | Chithunzi cha CTGTTGTAA |
| D594 | TTCTCAGCCA | TGGCTGAGAA |
| D595 | Mtengo wa TTGCATGGCT | AGCCATGCAA |
| D596 | Chithunzi cha TTGCGGTAC | GTACCGCCAA |
| Library Multiplex Primer (E-series) | Kwa Sampndi Mapepala MiSeq | Kwa Sampndi Mapepala NovaSeq, NovaSeq X, NextSeq, MiniSeq |
| E501 | AGCCCGGTC | Chithunzi cha GACCGTGGCT |
| E502 | Chithunzi cha CCGCCTGAGT | Chithunzi cha ACTCAGGCGG |
| E503 | GTTATATACC | GGATATAAC |
| E504 | Chithunzi cha CCTCTGGCA | Mtengo wa TGCCAGGAAG |
| E505 | Chithunzi cha ATCGAATTGC | Mtengo wa GCAATTCGAT |
| E506 | Chithunzi cha CACGTGTTGT | ACAACACGTG |
| E507 | Chithunzi cha GAGCTGTGTT | AACCAGCTC |
| E508 | Mtengo wa TATTACGAT | ACTGTAATA |
| E509 | Chithunzi cha ACAATGGCG | Mtengo wa CGCATTGTT |
| E510 | Chithunzi cha CAACTCGCTA | TAGMtengo wa CGAGTTG |
| E511 | Mtengo wa GAATTGCCTA | Mtengo wa TGAGCAATTC |
| E512 | GTGTGTTCGA | Mtengo wa TCGAACACAC |
| E513 | Mtengo wa CGATTCGAGC | GCTCGAATCG |
| E514 | Chithunzi cha GTCAACGTTA | Mtengo wa TAAGTTGAC |
| E515 | GACAATCCTA | TAGMtengo wa GATTGTC |
| E516 | Mtengo wa TATCAGACT | AGTCTGATTA |
| E517 | AAGCTTATAT | Zotsatira ATATAAGCTT |
| E518 | CATATTGTAA | Mtengo wa TTACATATG |
| E519 | GATAGMtengo wa GACTT | Chithunzi cha AAGTCCTATC |
| E520 | Mtengo wa ATGCTAGGTT | Mtengo wa AACCTAGCAT |
| E521 | Mtengo wa GCAAGATTAG | Chithunzi cha CTAATCTTGC |
| E522 | Chithunzi cha CAGCTGTATA | Mtengo wa TATACAGCTG |
| E523 | Mtengo wa GCAAGTCTAA | TTAGMtengo wa ACTTGC |
| E524 | TACTAGCAAG | Mtengo CTTGCTAGTA |
| E525 | AATACTGTTA | Mtengo wa TAACAGTATT |
| E526 | Zotsatira CATATCTGAC | GTCAGATATG |
| E527 | Mtengo wa GCCACTAAGG | Mtengo CCTTAGMtengo wa TGGC |
| E528 | Mtengo wa CTGCAATTC | Mtengo wa GAATTGCCAG |
| E529 | ACAATAGGCG | CGCCTATTGT |
| E530 | Mtengo wa CCATGCTAAG | CTTAGZotsatira CATGG |
| E531 | GCGAGCGATC | GATCGCTCGC |
| E532 | TAGGTAGMtengo wa TTC | GAACTACTA |
| E533 | Mtengo wa ACCTTGGACC | GGTCCAAGGT |
| E534 | Mtengo wa TGACTAATAC | GTATTAGMtengo wa TCA |
| E535 | GCTATATCTG | CAGATATAGC |
| E536 | TCTAGMtengo wa TTCAG | Mtengo wa CTGAACTAGA |
| E537 | ACGGTTGAGA | Chithunzi cha TCTCAACCGT |
| E538 | Chithunzi cha CTGATTCCGG | Zotsatira CCGGAATCAG |
| E539 | Mtengo wa TGGTAACAG | Chithunzi cha CTGGTTACCA |
| E540 | GACTCAGACA | Mtengo wa TGTCTGAGTC |
| E541 | TTAGCCAATA | Chithunzi cha TATTGGCTAA |
| E542 | Chithunzi cha CCTAAGGCG | Mtengo wa CGCTTAAGG |
| E543 | CGTAGZotsatira CAATC | GATTGCTACG |
| E544 | Malingaliro a kampani CATGGCTAAT | ATTAGMtengo wa CCATG |
| E545 | Mtengo wa ACTCACAAT | ATTGTCGAGT |
| E546 | Mtengo wa CGAGTCTAGG | CCTAGACTCG |
| E547 | Mtengo wa GGACTCAGCT | Mtengo wa AGCTGAGTCC |
| E548 | TCAGCAAGGA | Mtengo wa TCCTTGCTGA |
| E549 | Chithunzi cha CGATTCTTA | Mtengo wa TAAGGAATCG |
| E550 | ATTAGMtengo wa GCAAT | Mtengo wa ATTGCCTAAT |
| E551 | Mtengo wa TTGTCGAAGG | Chithunzi cha CCTTCGACAA |
| E552 | Mtengo wa TCCAAGTAGC | Zotsatira GCTACTTGGA |
| E553 | AGATAGTCCG | Mtengo wa CGGACTATCT |
| E554 | Mtengo CGCTAACATA | Mtengo wa TATGTTAGCG |
| E555 | Chithunzi cha GGCTAATTGT | Mtengo wa ACAATTAGCC |
| E556 | Mtengo wa AGGTCGTCA | Mtengo wa TGACGTACCT |
| E557 | TTCCAAGTTC | GAACTTGGAA |
| E558 | CGGAGCTCCG | CGGAGCTCCG |
| E559 | GGGATAATA | TATTAGMtengo wa TACC |
| E560 | TCGAAGGCAA | TTGCCTTCGA |
| E561 | Chithunzi cha CTCCTTGATG | Zotsatira CATCAGAG |
| E562 | Chithunzi cha GCAATTCCGG | Chithunzi cha CCGGAATTGC |
| E563 | Mtengo wa ACTACCAGC | Zithunzi za GCTGCGTAGT |
| E564 | Mtengo wa AGGAATGGCC | Mtengo wa GGCCATTCCT |
| E565 | Mtengo wa AGGTTAGCAT | Zotsatira ATGCTAACCT |
| E566 | Mtengo CGTCCTAGAC | GTCTAGMtengo wa GACG |
| E567 | Chithunzi cha GCGTGCAAT | ATTGCCACGC |
| E568 | Mtengo wa TCTGATCGAC | GTCGATCAGA |
| E569 | AGTTGTCAGG | Zotsatira CCTGACAACT |
| E570 | Mtengo wa CTACGCCAAT | Chithunzi cha ATTGCGTAG |
| E571 | GTAGChithunzi cha TTGCGT | Mtengo wa ACGCAACTAC |
| E572 | Mtengo wa TGAGCGCTAT | ATAGMtengo wa CGCTCA |
| E573 | Zotsatira za ATACCTTAGT | Chithunzi cha ACTAAGGTAT |
| E574 | GCACCATT | AATGGTGTGC |
| E575 | Zotsatira GTCTAAGGAG | Mtengo wa magawo CTCCTTAGAC |
| E576 | Chithunzi cha CATTCCCGG | Chithunzi cha CCGCGGAATG |
| E577 | Mtengo wa GCGTACCTAG | CTAGZithunzi za GTACGC |
| E578 | Mtengo CTCAATAGCA | Mtengo wa TGCTATTGAG |
| E579 | Chithunzi cha GTGCTAAGCC | Mtengo wa GGCTTAGMtengo CAC |
| E580 | Mtengo wa TGCCACTATC | GATAGMtengo wa TGGCA |
| E581 | Zotsatira GCTGTTGAAT | Mtengo wa ATTCAACAGC |
| E582 | Mtengo wa CGGCTAATCC | GGATTAGCCG |
| E583 | AACTACCGCA | Chithunzi cha TGCGGTAGTT |
| E584 | Zotsatira ATGCATCAAG | Chithunzi cha CTTGATGCAT |
| E585 | Mtengo wa CTGACTTGCA | Mtengo wa TGCAAGTCAG |
| E586 | Mtengo wa TGGTACTAT | ATAGGTACCA |
| E587 | ATTAGMtengo CTAAC | GTTAGZotsatira CTAAT |
| E588 | Mtengo wa TGGTACTAT | ATAGGTACCA |
| E589 | Mtengo wa TGTTGCTACC | GGTAGCACA |
| E590 | Zotsatira CTTATATAAG | Zotsatira CTTATATAAG |
| E591 | TCAAGTCTGT | ACAGACTTGA |
| E592 | Mtengo wa TGAGCGCTAT | ATAGMtengo wa CGCTCA |
| E593 | TTAGGCGTAC | GTCGCCTAA |
| E594 | Mtengo wa TCTCATCAG | CTGATGAGAA |
| E595 | CTAGZithunzi za GTATTG | CAATACCTAG |
| E596 | Mtengo wa TTGCTGACTA | TAGMtengo wa TCAGCAA |
| Library Multiplex Primer (F-series) | Kwa Sampndi Mapepala MiSeq | Kwa Sample Mapepala NovaSeq, NovaSeq X, Next Seq, MiniSeq |
| F501 | Mtengo wa AGCCAGTAGC | Mtengo wa GCTACTGGCT |
| F502 | Chithunzi cha CCGATGAGTT | Mtengo wa AACTCATCGG |
| F503 | Mtengo wa GTTCAACTTC | Mtengo wa GAAGTTGAAC |
| F504 | TTGAGCATCA | TGATGCTCAA |
| F505 | Chithunzi cha ATCACAGTG | Zotsatira CACTGTTGAT |
| F506 | Mtengo wa CACACGGTAG | Chithunzi cha CTACCGTGTG |
| F507 | Gawo la GGATTGCT | Mtengo wa AGCAATCCTC |
| F508 | Chithunzi cha TATCTTGGCC | GGCCAAGATA |
| F509 | AACTAGMtengo wa TGGC | Mtengo wa GCCACTAGTT |
| F510 | Chithunzi cha CAATCTGGT | ACCAGGATTG |
| F511 | GAAGGTTACG | Mtengo wa CGTAACCTTC |
| F512 | Mtengo wa CTAAGAACCT | Mtengo wa AGGTCTTAG |
| F513 | Mtengo wa CACACGGTAG | Chithunzi cha CTACCGTGTG |
| F514 | AATTAGMtengo wa TCTG | Chithunzi cha CAGACTAATT |
| F515 | GACCGTGTTA | Mtengo wa TAACCGGTC |
| F516 | Chithunzi cha TAATTCGAGG | Mtengo wa CCTCGAATTA |
| F517 | Chithunzi cha AAGATTGACG | CGTCAATCTT |
| F518 | Chithunzi cha CAACTCGCTA | TAGMtengo wa CGAGTTG |
| F519 | Zotsatira GATTCGAACT | AGTTCGAATC |
| F520 | Mtengo wa GCCTTGAGTT | Mtengo wa AACTCAAGGC |
| F521 | CACAGATTAG | Mtengo wa CTATCTGTG |
| F522 | CAGCTAGMtengo wa GCC | Mtengo wa GGCCTAGMtengo wa CTG |
| F523 | Mtengo wa GCAAGGTATA | TATACCTTGC |
| F524 | Mtengo wa TACCTAGGTA | Mtengo wa TACCTAGGTA |
| F525 | AATCCTTGAA | Mtengo wa TTCAAGGATT |
| F526 | Chithunzi cha CATCGAACCT | AGGTTCGATG |
| F527 | GCCTGCGTAA | TTACGCAGGC |
| F528 | Chithunzi cha CATCGAACCT | AGGTTCGATG |
| F529 | Mtengo wa ACAGTGCCAT | Chithunzi cha ATGGCACTGT |
| F530 | Zotsatira CCAACTATTG | CAATAGMtengo wa TTGG |
| F531 | GCGTACCGCA | Chithunzi cha TGCGGTACGC |
| F532 | TAGChithunzi cha GAGCGAT | Chithunzi cha ATCGCTCCTA |
| F533 | ACCAATTGGT | ACCAATTGGT |
| F534 | AATACTGTTA | Mtengo wa TAACAGTATT |
| F535 | Zotsatira GCTGTTGAAT | Mtengo wa ATTCAACAGC |
| F536 | Mtengo wa TTACAACCAG | Chithunzi cha CTGTTGTAA |
| F537 | Mtengo wa ACGTCAAGTC | GACTTGACGT |
| F538 | Chithunzi cha CATTCAATG | Zotsatira CATTGAATTG |
| F539 | Mtengo wa TGAATTCAGC | Mtengo wa GCTGAATTCA |
| F540 | ACGTCAGCT | Mtengo wa AGCTGTACGT |
| F541 | Mtengo wa TGACTACCAT | Mtengo wa ATGGTAGMtengo wa TCA |
| F542 | Chithunzi cha CCTGGTGA | Mtengo wa TCCACCAGG |
| F543 | GACTAATCGC | Mtengo wa GCGATTAGTC |
| F544 | ACGTTAGTGC | Mtengo wa GCACTAACGT |
| F545 | ACTCGATCT | AGAATCGAGT |
| F546 | CGACAAGATT | AATCTTGTCG |
| F547 | GGAATTGGCT | AGCCAATTCC |
| F548 | TCACAGCCGA | Mtengo wa TCGGCTGA |
| F549 | Mtengo wa CTGTACTGTA | Malingaliro a kampani TACAGTACAG |
| F550 | Mtengo wa TCGCTCGAGG | CCTCGAGCGA |
| F551 | ACCTTAGCAA | Chithunzi cha TTGCTAAGGT |
| F552 | Mtengo wa TCCGGACTAC | GTAGTCCGGA |
| F553 | AGAACTTGCA | Mtengo wa TGCAAGTTCT |
| F554 | Mtengo wa CGAGGACTT | AAGTCCTGCG |
| F555 | GGCAAGATGA | Mtengo wa TCATCTTGCC |
| F556 | Mtengo wa GCCTAGCGCG | Mtengo CGCGCTAGGC |
| F557 | GACATCGCTA | TAGMtengo wa CGATGTC |
| F558 | Mtengo wa CGGCTAATCC | GGATTAGCCG |
| F559 | GGTTGAGCTT | AAGCTCAACC |
| F560 | TCGCTCAGGC | GCCTGAGCGA |
| F561 | GCTGATTATA | TATAATCAGC |
| F562 | GCGCTAGTCC | Mtengo wa GGACTAGCGC |
| F563 | TATGTCTGAA | TTCAGACATA |
| F564 | AAGCTTATAT | Zotsatira ATATAAGCTT |
| F565 | Mtengo wa AGGAATGGCC | Mtengo wa GGCCATTCCT |
| F566 | CGTAGMtengo CGCGC | GGCGCTACG |
| F567 | GCCTGCGTAA | TTACGCAGGC |
| F568 | Mtengo wa TCTTAGCGAT | ATCGCTAAGA |
| F569 | AGTTCTAAGA | Mtengo wa TCTTAGAACT |
| F570 | CTAGZithunzi za GTATTG | CAATACCTAG |
| F571 | GTAGMtengo wa TACTAC | GTAGMtengo wa TACTAC |
| F572 | Mtengo wa TGACAAGTAG | Mtengo wa CTACTTGTCA |
| F573 | ATAGMtengo wa TCTGAG | Zotsatira CTCAGACTAT |
| F574 | Mtengo wa CTGTGTTACA | Mtengo wa TGTAACACAG |
| F575 | GTCTAGCCAC | Chithunzi cha GTGGCTAGAC |
| F576 | Chithunzi cha AATCAAGGTT | Chithunzi cha AACCTTGATT |
| F577 | Chithunzi cha CATAATTATG | Chithunzi cha CATAATTATG |
| F578 | Chithunzi cha CTCGAGCTAT | ATAGMtengo wa CTCGAG |
| F579 | Chithunzi cha GTGCCTGATG | Chithunzi cha CATCAGGCAC |
| F580 | Chithunzi cha TGCTCGATTG | CAATCGAGCA |
| F581 | Zotsatira GATTCGAACT | AGTTCGAATC |
| F582 | Mtengo wa TCGTTGAGGA | Mtengo wa TCCTCAACGA |
| F583 | AGCCAGGCGT | ACGCCTGGCT |
| F584 | Chithunzi cha ATGTCAGATT | Chithunzi cha AATCTGACAT |
| F585 | Mtengo wa CTGATATAC | Chithunzi cha GATATACAG |
| F586 | Chithunzi cha TGGTCAAC | GTTGACACCA |
| F587 | Mtengo wa ATTGCATGCT | Malingaliro a kampani AGCATGCAAT |
| F588 | AGTATTCATA | Chithunzi cha TATGAATACT |
| F589 | TGTAATTCCG | CGGAATTACA |
| F590 | Chithunzi cha CCTCTGGCA | Mtengo wa TGCCAGGAAG |
| F591 | TCCGCGGA | TCCGCGGA |
| F592 | Chithunzi cha CACTGTCGG | CCGAACAGG |
| F593 | TTAGCCAATA | Chithunzi cha TATTGGCTAA |
| F594 | Mtengo wa TTCGAGCTGA | TCAGCTCGAA |
| F595 | GTACCCGCG | CGCGCGGTAC |
| F596 | TTGAGCATCA | TGATGCTCAA |
Zowonjezera
Kuthamangitsa TELL-Seq™ Custom Primers mu Illumina® Sequencing Primers
Malaibulale a TELL-Seq™ amafunikira zoyambira zotsatirira pamapulatifomu a Illumina. Spiking (kapena kuphatikiza) makonda a TELL-Seq™ Illumina kutsata zoyambira muzoyambira zoyambira za Illumina ndikofunikira mukaphatikiza laibulale yoyang'anira ya PhiX kapena malaibulale ena wamba a Illumina okhala ndi malaibulale a TELL- Seq™ motsatizana.
Zindikirani: TELL-Seq™ Index 1 yowerenga ili ndi 18-base ndipo ikufunika kutsatizana kwa 18-cycle; Kuwerenga kwa index 2 kumakhala ndi 8-base kwa T-series primer ndi 10-base kwa C-series primer.
Njira yopangira zoyambira zoyambira muzoyambira za Illumina
- Malo a cartridge, ma voliyumu onse, ndi kuchuluka komaliza kwa zoyambira papulatifomu iliyonse zaperekedwa m'matebulo omwe ali pansipa.
- Werengerani kuchuluka kwa choyambira chachizolowezi kuti muwonjezere malo a cartridge ya Illumina potengera kuchuluka komaliza kwa choyambira chomwe chili mu katiriji.
- Pambuyo pokoka muzoyambira, sinthani pipette kukhala theka la voliyumu yonseyo ndipo pang'onopang'ono pipette mmwamba ndi pansi kasanu kuti musakanize bwino.
- Kwa MiSeq, MiniSeq, NextSeq ndi NovaSeq nsanja, musayang'ane bokosi la "custom primer" mu s.ample sheet kapena panthawi yokonzekera.
* Kuti muwerenge kuchuluka kwa zoyambira zomwe zingadutse mu chitsime, gwiritsani ntchito (C1)*(V1) = (C2)*(V2) equation pomwe:
- C1 = 100μM (pamene mukugwiritsa ntchito 100μM high concentration primer stock, voliyumu yaying'ono yowonjezera voliyumu yomaliza ndi yosafunika).
- V1 = thetsani kuti voliyumu yoyambira yoyambira ilowedwe
- C2 = zoyambira zoyambira zomwe zimalimbikitsidwa kuchokera pa tchati chomwe chili pansipa V2 = kuchuluka kwathunthu kwa Illumina primer pama chart omwe ali pansipa
Example kwa nsanja ya MiSeq
100μM * V1 = 0.5μM * 680μL V1 = 3.4 µl
Chidziwitso chofunikira: Malangizo omwe ali pansipa akutengera ma voliyumu oyambira pano. Kuti muwonetsetse kulondola, yesani ma voliyumu oyambira mu katiriji ndi pipette musanayambe kukhazikitsa.
Sequencer iliyonse ya Illumina® imakhala ndi tsatanetsatane womaliza komanso kuchuluka kwa voliyumu kotero onani zomwe zili pansipa kuti muwonetsetse kuti kuchuluka koyenera kwa TELL-Seq™ Illumina® Sequencing Primers ikuwonjezedwa mwachindunji.
*Zindikirani: Mlozera 1 ndi Mlozera 2 pamodzi ndi Werengani 1 ndi Werengani 2 zoyambira nthawi zambiri zimaphatikizidwa mu cartridge ya reagent bwino. Chonde onetsetsani kuti choyambira chomaliza chachitsime chikhala choyambira chilichonse (monga 0.3μM ya Index 1 ndi Index 2 yokwana 0.6μM)
<https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000001542>
M'munsimu muli
iSeq100
Chifukwa cha mapangidwe a cartridge ya iSeq100, sizingatheke kukweza ndi kugwiritsa ntchito zoyambira zoyambira.
MiniSeq
NextSeq
MiSeq
* Palibe njira yopangira chizolowezi cha Index 2 (i5) popeza template imagwiritsa ntchito P5 primer pamwamba pa cell cell.
HiSeq 1000/2000 - 1500/2300 
* Palibe njira yopangira chizolowezi cha Index 2 (i5) popeza template imagwiritsa ntchito P5 primer pamwamba pa cell cell.
HiSeq 3000/4000
NovaSeq v1.0 Consumables
| Mtundu wa kit | Woyamba wa Ilumina (dzina) | Malo a Cartridge | Zonse Voliyumu (mL) | Mwambo choyambirira ndende yomaliza (µM) |
|
SP 100/200/300/500 zozungulira |
Werengani 1 (BP10) | 24 | 4 | 0.3 |
| Mlozera 1 (i7) (BP14)* | 23 | 5 | 0.3 | |
| Werengani 2 (BP11) | 13 | 2 | 0.3 | |
|
S1 ndi S2 100/200/300 kuzungulira |
Werengani 1 (BP10) | 24 | 4 | 0.3 |
| Mlozera 1 (i7) (BP14)* | 23 | 5 | 0.3 | |
| Werengani 2 (BP11) | 13 | 2 | 0.3 | |
|
S4 200/300 kuzungulira |
Werengani 1 (BP10) | 24 | 7.3 | 0.3 |
| Mlozera 1 (i7) (BP14)* | 23 | 5 | 0.3 | |
| Werengani 2 (BP11) | 13 | 3.5 | 0.3 |
* Palibe njira yopangira chizolowezi cha Index 2 (i5) popeza template imagwiritsa ntchito P5 primer pamwamba pa cell cell.
NovaSeq v1.5 Consumables
| Mtundu wa kit | Illumina Primer (dzina) | Malo a Cartridge | Zonse Voliyumu (mL) | Mwambo choyambirira ndende yomaliza (µM) |
|
SP 100/200/300/500 zozungulira |
Werengani 1 (VP10) | 24 | 4 | 0.3 |
| Mlozera 1 (i7) ndi Mlozera 2 (i5) (VP14) | 23 | 5 | 0.3 | |
| Werengani 2 (VP11) | 13 | 2 | 0.3 | |
|
S1 ndi S2 100/200/300 kuzungulira |
Werengani 1 (VP10) | 24 | 4 | 0.3 |
| Mlozera 1 (i7) ndi Mlozera 2 (i5) (VP14) | 23 | 5 | 0.3 | |
| Werengani 2 (VP11) | 13 | 2 | 0.3 | |
|
S4 200/300 kuzungulira |
Werengani 1 (VP10) | 24 | 7.3 | 0.3 |
| Mlozera 1 (i7) ndi Mlozera 2 (i5) (VP14) | 23 | 5 | 0.3 | |
| Werengani 2 (VP11) | 13 | 3.5 | 0.3 |
NovaSeq X/X Plus
Kukhazikitsa TELL-Seq™ Kuthamanga pa NextSeq™ 1000/2000 System
Katiriji ya NextSeq™ 1000/2000 reagent ili ndi zitsime ziwiri (1 ndi 2, zonse zilibe kanthu) kuti zigwiritsidwe ntchito laibulale imafuna choyambira chimodzi. Imathandizira mpaka zoyambira ziwiri zoyambira (dziwe) pachitsime chilichonse. Ndikofunikira kuti Read primer ndi Index primer zili m'zitsime zosiyanasiyana.
Ma library a TELL-Seq™ amafunikira zoyambira zowerengera zonse (werengani 1, werengani 2, index 1 ndi index 2). Pomwe malaibulale a TELL-Seq™ okha ndi omwe amatsatiridwa motsatizana
- Phatikizani TELL-Seq™ werengani 1 ndikuwerenga zoyambira 2: gwiritsani ntchito HT1 kuti muchepetse kusakaniza kulikonse kowerengera kuti mupereke 600 µl pa 0.3 µM komaliza, mwachitsanzo, 1.8 µl ya 100 µM TELL-Seq™ werengani 1 choyambirira ndi 1.8 µl ya 100 µl ya 2. µM TELL-Seq™ werengani 597 koyambirira Mtengo wa 1μl HT1. Kwezani mu makonda bwino XNUMX.
- Phatikizani TELL-Seq™ index 1 ndi index 2 primers: gwiritsani ntchito HT1 kuti muchepetse kusakaniza koyambirira kwa 600 µl pa 0.6 µM komaliza, mwachitsanzo, 3.6 µl ya 100 µM TELL-Seq™ index 1 yoyambira ndi 3.6 µl ya 100 µl ya µM TELL-Seq™ index 2 choyambirira kulowa Mtengo wa 593µl HT1. Kwezani mu makonda bwino 2.
- Sankhani choyambira choyenera bwino mukakhazikitsa njira yotsatirira motere:
- Werengani 1: Mwambo 1
- Mlozera 1: Mwamakonda 2
- Mlozera 2: Mwamakonda 2
- Werengani 2: Mwambo 1
Pamene malaibulale a PhiX amagwiritsidwa ntchito ndi malaibulale a TELL-Seq™ kuti atsatire motsatizana
- Pezani NextSeq 1000/2000 Werengani Primer Kit (Catalog # 20046117)
Phatikizani TELL-Seq™ werengani 1 ndikuwerenga zoyambira 2 mu HP21: Onjezani kusakaniza kowerengera koyambira ku 600 µl HP21 kwa 0.3 µM komaliza, mwachitsanzo, 1.8 µl ya 100 µM TELL-Seq™ werengani choyambirira 1 ndi 1.8 µl ya 100 µl ya 2. µM TELL-Seq™ werengani 597 koyambirira 21µl HP1. Kwezani mu makonda bwino XNUMX.- Phatikizani TELL-Seq™ index 1 ndi index 2 primers: gwiritsani ntchito HT1 kuti muchepetse kusakaniza koyambirira kwa 600 µl pa 0.6 µM komaliza, mwachitsanzo, 3.6 µl ya 100 µM TELL-Seq™ index 1 yoyambira ndi 3.6 µl ya 100 µl ya µM TELL-Seq™ index 2 choyambirira kulowa Mtengo wa 593µl HT1. Kwezani mu makonda bwino 2.
- Sankhani choyambira choyenera bwino mukakhazikitsa njira yotsatirira motere:
- Werengani 1: Mwambo 1
- Mlozera 1: Mwamakonda 2
- Mlozera 2: Mwamakonda 2
- Werengani 2: Mwambo 1
Pamene malaibulale apawiri a Illumina akutsatiridwa ndi malaibulale a TELL-Seq™ pamodzi mothamanga.
- Pezani NextSeq 1000/2000 Read & Index Primer Kit (Catalog# 20046115)
- Phatikizani TELL-Seq™ werengani 1 ndikuwerenga zoyambira 2 mu HP21: Onjezani kusakaniza kowerengera koyambira ku 600 µl HP21 kwa 0.3 µM komaliza, mwachitsanzo, 1.8 µl ya 100 µM TELL-Seq™ werengani choyambirira 1 ndi 1.8 µl ya 100 µl ya 2. µM TELL-Seq™ werengani 597 koyambirira 21µl HP1. Kwezani mu makonda bwino XNUMX.
- Phatikizani TELL-Seq™ index 1 ndi index 2 primer mu BP14: Onjezani kusakaniza koyambirira kwa 600 µl BP14 kwa 0.6 µM komaliza, mwachitsanzo, 3.6 µl ya 100 µM TELL-Seq™ index 1 primer ndi 3.6l ya µM TELL-Seq™ index 100 choyambirira kulowa 2µl BP593. Kwezani mu makonda bwino 14.
- Sankhani choyambira choyenera bwino mukakhazikitsa njira yotsatirira motere:
- Werengani 1: Mwambo 1
- Mlozera 1: Mwamakonda 2
- Mlozera 2: Mwamakonda 2
- Werengani 2: Mwambo 1
Zolemba / Zothandizira
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Universal Sequencing TELL-Seq Library [pdf] Buku Logwiritsa Ntchito TELL-Seq Library, TELL-Seq, Library |
