IZON TN-DQ-012 qEVSINGLE Smart Columns User Guide

IZON TN-DQ-012 qEVSINGLE Smart Columns

MALANGIZO OYambira GUZANI

KWA qEVSINGLE (35nm & 70nm) COLUMNS SMART Maupangiri oyambira mwachanguwa amangopereka malangizo ogwiritsira ntchito. Kuti mumve zambiri, mutha kutsitsa laibulale yonse ya qEV User Manuals and Technical Notes kuchokera pa Izon support portal. Mapepala a Chitetezo akupezeka pa izon.com/sds

NTCHITO YOTSATIRA

Mizati ya Izon qEV SMART imapatula ma vesicles owonjezera kuchokera ku biological samples. Mizati ya qEV SMART ili ndi tchipisi ta RFID kuti tigwiritse ntchito ndi Izon Automatic Fraction Collector (AFC). Ma chips awa sangakhudze kugwiritsa ntchito pamanja. Gawo la qEV lakonzedwa kuti ligwiritsidwe ntchito m'ma laboratories ofufuza ndi akatswiri kuti agwiritse ntchito kafukufuku kokha. Gawo la qEV silinapangidwe kuti liziwunikira matenda ndipo siliyenera kugwiritsidwa ntchito popanga chisankho chamankhwala.

MALANGIZO OGWIRA NTCHITO

1.  Centrifuge samppang'ono asanakweze ndime kuti achotse ma cell ndi zinyalala zazikulu zama cell. Poyamba centrifuge pa 1500 xg kwa mphindi 10 kuchotsa maselo aliwonse ndi tinthu tating'onoting'ono. Rencentrifuge the supernatant pa 10,000 xg kwa mphindi 10. Pakupatula ma microvesicle, gwiritsani ntchito mphamvu zochepa za g pa sitepe yachiwiri ya centrifugation.
2.  Kwa voliyumu yayikulu samples, n'zotheka kuika maganizo a sample musanakweze pagawo la qEV. Izi sizikugwira ntchito pa seramu ndi plasmaampomwe ali ndi mapuloteni ochuluka kwambiri. Izon amalimbikitsa kugwiritsa ntchito zida za Merck Millipore, zosefera za Amicon® Ultra Centrifugal.
3.  qEVsingle columns amalimbikitsidwa kuti agwiritse ntchito kamodzi kokha.
4.  Onetsetsani kuti sampLe buffer ndi kutentha komweko monga gawoli
   (makamaka pakati pa 18-24 ˚C).
5. Gwiritsani ntchito buffer yatsopano (0.22 µm) kuti mupewe kuipitsidwa.

KULINGALIRA MALANGIZO OGWIRITSA NTCHITO

1.  Gwirizanitsani ndime ndi sampChosungiracho chiyenera kukhala mkati mwa kutentha kwa 18-24 ˚C. Osachotsa zipewa mpaka kutentha kwa ntchito kufikire.
2.  Chotsani mosamala chipewa chapamwamba chokha.
3.  Ikani mzatiwo mowongoka pa choyikapo chokonzekera kugwiritsidwa ntchito. Ma qEV Racks ndi Automatic Fraction Collectors akupezeka ku Izon Science.
4. Onjezani mzere ndi buffer.

KUPULUKA KWA NTHAWI

1. Chotsani kapu yapansi ndikulola kuti buffer iyambe kudutsa ndime.
2. Yatsani gawoli ndi gawo limodzi la buffer. Ngati mapulogalamu anu akumunsi akhudzidwa ndi sodium azide, tsitsani ndi ma voliyumu awiri a bafa. Ngati chotchinga chotchinga china kupatula PBS ndichoti chigwiritsidwe ntchito, gwirizanitsani gawoli ndi magawo atatu a bokosi latsopanolo. Chigawocho chidzasiya kuyenda chokha pamene buffer yonse yalowa mu frit yotsegula.

MANUAL SAMPLE KUSONYEZA

1.  Sefa kapena centrifuge biological sample kuchotsa zinthu zazikulu. Onaninso malangizo ogwiritsira ntchito.
2. Buffer ikasiya kuyenderera muzakudya kuti isasunthike, kwezani ma centrifuged okonzekaamplembani voliyumu ku frit yotsitsa.
3.  Nthawi yomweyo yambani kutolera buffer volume1 (izi zikuphatikiza sampndi volume).
4. Lolani sample kuthamangira mu column. Mzere udzasiya kuyenda pamene onse a sample walowa mu loading frit.
5.  Kwezani mgawo ndi buffer ndikupitiliza kusonkhanitsa voliyumu ya bafa.
6. Voliyumu ya buffer ikasonkhanitsidwa, pitilizani kusonkhanitsa Purified Collection Volume (PCV)2. Onani Zithunzi 1 & 2.
7. Kuti mutenge ma voliyumu olondola, ingokwezani voliyumu yomwe ikufunika pamwamba pa ndime, dikirani kuti voliyumu idutse mpaka kutuluka kutayima ndikubwereza. Pewani kuyimitsa mzati pakuyenda kwa nthawi yayitali kuti muwonetsetse kulekanitsidwa kolondola kwa EV.

ZOCHITIKA

Sampndi Load Volume		≤ 0.150 mL
Column Volume		3.5 mL
Buffer Volume	qEV/35	0.8 mL
	qEV/70	1.0 mL
Kukula Kwagawo Loyenera		0.2 mL
Buffer yofunikira pa sample zosonkhanitsira		2.0 mL

1. Voliyumu ya buffer - voliyumu ya buffer yomwe imachoka pamndandanda pomwe tinthu tating'onoting'ono ta chidwi.
2. Purified Collection Volume (PCV) - voliyumu yoyeretsedwa ndi gawo lomwe lili ndi tinthu tating'onoting'ono ta chidwi.

Zolemba / Zothandizira

IZON TN-DQ-012 qEVSINGLE Smart Columns [pdf] Wogwiritsa Ntchito TN-DQ-012 qEVSINGLE Smart Columns, TN-DQ-012, qEVSINGLE Mizati Yanzeru, Mizati Yanzeru, Mizere

Zothandizira

• Izon Science Safety Data Sheets (SDS)
• Dzipatulani & Muyese Zowonjezera Zowonjezera & Nanoparticles