IZON AFC(V1) Automated Size Exclusion Chromatography User Guide

IZON AFC(V1) Automated Size Exclusion Chromatography

MALANGIZO

CHOFUNIKA - Werengani musanagwiritse ntchito zida za Izon

Kugwiritsiridwa ntchito kwa chida ichi m'njira yosatchulidwa ndi malangizo omwe ali mu bukhuli kungawononge chitetezo choperekedwa ndi / kapena kusokoneza ntchito kapena kuwonongeka kwa chida. Osagwiritsa ntchito chipangizocho kunja kwa voliyumu yake yoperekedwatages kapena chilengedwe.

Werengani chikalata chonse cha ogwiritsa ntchito cha AFC(V1) musanatulutse zida zanu za Izon kapena kutsatira njira zomwe zili pansipa.
Buku lathunthu la AFC(V1) likupezeka pa support.izon.com/afc-user-manual

Kugwiritsa Ntchito
AFC(V1) imagwiritsidwa ntchito kupanga tongoleredwe kagawo ka Izon's qEV size-exclusion columns.
Chidachi chimapangidwa kuti chigwiritsidwe ntchito m'ma laboratories ofufuza ndi akatswiri kuti agwiritse ntchito kafukufuku kokha. Ngakhale gawo la AFC(V1) kapena gawo la qEV silinagwiritsidwe ntchito popanga zisankho zachipatala.
Kuti mutsimikizire dongosolo lonselo, tikulimbikitsidwa kuti muzitsatiridwa ndi Good Laboratory Practices (GLP) kuti mutsimikizire kusanthula kodalirika.

Misonkhano Yachigawo
Onetsetsani kuti mukutsata mfundo zodzitetezera zomwe zaperekedwa mu bukhuli. Chitetezo ndi zidziwitso zina zapadera zidzawonekera m'mabokosi ndikuphatikiza zomwe zalembedwa mu Gulu 1:

	Chizindikirochi chikuwonetsa upangiri wanthawi zonse wamomwe mungasinthire njira kapena kupangira njira zomwe mungatsatire pazochitika zinazake
	Chizindikirochi chimasonyeza kumene tiyenera kusamala kwambiri
	Mawu omwe amawonekera mu pinki amagwiritsidwa ntchito kuwonetsa mabatani ndi machitidwe omwe amagwiritsidwa ntchito pazithunzi za AFC(V1)

MALO OGWIRITSIRA NTCHITO

Gawo 1A: Kapangidwe ka Zida
Zigawo zotsatirazi zaperekedwa m'bokosi.

Zigawo za AFC(V1) System:

1. Chida cha AFC(V1) chokhala ndi Nozzle Set ndi Silicone Waste Tube
2. Seti ya Nozzle (x3)
3. EV Column Mount (x5)
4. Chophimba cha Carousel
5. Carousel Wosinthika
6. Carousel Plate
7. EVoriginal Reservoir
8. 5.0 mL Eppendorf Tube (x15)
9. 2.0 mL Eppendorf Tube (x30)
10. Mphamvu ya 12-volt
11. Mtsogoleri Wamphamvu
12. USB 2.0 Type A to USB 2.0 Micro Cable (yachimuna kwa mwamuna)
13. 10 g Calibration Kulemera kwake

Gawo 1B: Mapangidwe a Carousel Yosinthika

Carousel ndi yosinthika ndipo itha kugwiritsidwa ntchito munjira ziwiri:

• Mbali imodzi ili ndi mabowo asanu ndi awiri ndipo imagwiritsa ntchito machubu otolera 5 ml. Mbali iyi ndi yogwiritsidwa ntchito ndi qEV10 ONLY.
• Mbali imodzi ili ndi mabowo ang'onoang'ono khumi ndi atatu ndipo imagwiritsa ntchito machubu otolera a 1.5mL kapena 2.0mL. Mbali iyi itha kugwiritsidwa ntchito ndi single, qEVoriginal, qEV1 ndi qEV2.
  Chithunzi 2 ndi Chithunzi 3 chikuwonetsa mawonekedwe a carousel yosinthika. Dziwani kuti bowolo liribe njira yopangira chivundikiro cha chubu ndipo liribe nambala yolumikizidwa.
  Kuphatikiza pa mabowo a machubu osonkhanitsira magawo, mbali zonse za carousel zili ndi izi:

  A	Central Well
  B	Central Well Channel
  C	Flush Hole

Gawo 1C: Zida Zazida Zomwe Zimafunikira Koma Zosaperekedwa

Zida zotsatirazi ndizofunika kuti zigwiritsidwe ntchito koma sizinaperekedwe:

• Kompyuta yokhala ndi Windows 10 OS yosinthira firmware ya AFC(V1).
• Chithunzi cha qEV.
• Pipettes ndi malangizo.
• Zokonzedwa kumene komanso 0.22 µm zosefera zosefera.
• Kukonzekera kwaposachedwa kwa 0.5 M NaOH yothetsera.
• 70% Ethanol njira yoyeretsera.

GAWO 2: KULIMBIKITSA CHIDA CHANU

Mphamvu ya Chida ndi Kayendetsedwe Kazonse

• Lumikizani kumalo otetezedwa okhala ndi nthaka okha.
• Onetsetsani kuti bokosi lamagetsi lili kutali ndi madzi.
• Kuti mupewe kutentha, musatseke bokosi lamagetsi.
• Position unit kuti itha kulumikizidwa mwachangu komanso mosavuta kumagetsi a mains.
• Onetsetsani kuti katundu wapafupi akukwaniritsa zofunikira za AC zomwe zaperekedwa mwatsatanetsatane.
  Zida za Izon ziyenera kugwiritsidwa ntchito ndi zida za Izon zoperekedwa ndi magetsi. Kukanika kugwiritsa ntchito magetsi olondola kungayambitse ntchito yolakwika.
  Onetsetsani kuti magetsi ayikidwa kutali ndi chidacho komanso kumbuyo kwa chipangizocho kuti musakhumane ndi kutaya kapena madzimadzi.

AFC(V1) CALIBRATION

Gawo 3A: Kuyanjanitsa Carousel
Chidacho chikasonkhanitsidwa pamtunda, carousel iyenera kulumikizidwa ndi nozzle yamadzimadzi.

1. Dinani SETUP kumanzere kwa sikirini yayikulu.
2. Kutsatira sikirini, sankhani CAROUSEL > CALIBRATE
3. Dinani -/+ mabatani (pofuna kuyanjanitsa) ndi -/++ mabatani (kuti muyanjanitse bwino) mpaka mphunoyo itayimitsidwa pamwamba pa dzenje lomwe limatuluka.
4. Bowolo likalumikizidwa ndi mphuno, dinani SET ZERO. Mudzabwereranso ku chophimba chachikulu chokonzekera.
   Kuwongolera kolakwika kwa carousel kungayambitse madontho kuchokera pamphuno kuti agwere pa carousel kapena kuphonya machubu otolera. Izi zikachitika, ingobwerezaninso carousel. Kuti mumve zambiri, chonde onani Kuwongolera Mavuto.

Gawo 3B: Kuwongolera Selo Yonyamula 

1. Kuchokera pa mndandanda waukulu, dinani SETUP > SALE > CALIBRATE, ndikutsatira malangizo omwe ali pa zenera.
2. Mukalangizidwa, ikani carousel yopanda kanthu pa AFC(V1) ndikusindikiza OK.
3. Kuunikira kudzasintha kukhala kofiira kusonyeza kuti chidacho chikuyeza kulemera kwa carousel.
   Njira yoyezera ikangoyamba, wogwiritsa ntchito sayenera kugunda kapena kusokoneza carousel mpaka kuyatsa kwasintha kukhala kobiriwira kusonyeza kuti ntchitoyo yatha. Kusokoneza kulikonse kudzafuna kuti wogwiritsa ntchito ayambenso kuwongolera.
4. Muyezo ukatha, kuyatsa kudzasintha kukhala kobiriwira ndipo chinsalu chidzalangiza wogwiritsa ntchito kuti ayike kulemera kwake kwapakati pa carousel yopanda kanthu.
5. Onetsetsani kuti kulemera kwake kudakalibe MUSANAKANIZE CHABWINO kuti mupitilize.
6. Kuunikirako kudzasintha kukhala kofiira kusonyeza kuti chidacho chikuyesa kulemera kwake.
7. Kuyesa kwa cell cell kukamalizidwa, wogwiritsa ntchito adzabwezeredwa kumenyu yokhazikitsira.
8. Dinani EXIT kuti mubwerere ku sikirini yayikulu.

NTCHITO YA AFC(V1)

Gawo 4A: Kukweza Column ku AFC(V1) Tower
AFC (V1) idzagwira ntchito ndi RFID-tagged anzeru qEV mizati. Musayese kugwiritsa ntchitotagged kapena sanali a Izon ndi chida cha AFC(V1).

1. Yatsani chidacho poyatsa batani lamphamvu kumbuyo kwa chidacho.
2. Yang'anani chubu la zinyalala kuti muwonetsetse kuti likulowa mu sink kapena chotengera cha zinyalala.
3. Mzere usanalowetsedwe kapena ngati ndime yopanda RFID tag yayikidwa, kuyatsa kwa nsanja kudzakhala koyera, ndipo chophimba chachikulu chidzawonetsa "Palibe Column". Chida cha AFC(V1) chimalemba kuchuluka kwa nthawi zomwe gawo lililonse lanzeru lagwiritsidwa ntchito. Mizatiyo idapangidwa kuti igwiritsidwe ntchito mpaka nthawi 5. Mzere udzakhala wopanda ntchito ukagwiritsidwa ntchito ka 10, ndipo chophimba chachikulu chidzawonetsa "Mzere Wosavomerezeka".
4. Konzani ndime yoyenera pansanja ya AFC(V1).
5. Ngati mukugwiritsa ntchito imodzi, qEVoriginal, kapena qEV1, chotsani chipewa chapamwamba musanachotse chipewa cham'munsi cha gawolo musanapite ku sitepe yotsatira. Ngati mukugwiritsa ntchito qEV2 kapena qEV10, ikani mosungiramo ndime ndipo onetsetsani kuti madziwo akuyenda bwino musanapitirire sitepe ina.
6. Lowetsani ndime kuchokera pamwamba mu khwekhwe, kenako chotsani kapu yapansi ndikuyika bwino gawolo. Onetsetsani kuti logo ya IZON yomwe ili pamndandandayo ikuyang'ana kutali ndi nsanja ya AFC(V1).
7. Tembenuzirani gawoli mkati mwa phirilo mpaka mtundu wa qEV womwe wagwiritsidwa ntchito uwonetsedwa pazenera.
8. Onetsetsani kuti kugwirizana pakati pa ndime ndi valavu ndi otetezeka kuti asatayike.
   Ndizofala kuti madontho ochepa a buffer atayike pamene akugwirizanitsa ndime ndi cholumikizira cha luer pa valve. Kuti muchepetse izi, pangani kulumikizana mwachangu ndipo onetsetsani kuti mwapukuta madontho aliwonse omwe agwera pachidacho.

Gawo 4B: Kusintha ndondomeko ya kusonkhanitsa

Gawo loyenera likasankhidwa ndikukwezedwa ku nsanja ya AFC(V1), ndondomeko yosonkhanitsa iyenera kukhazikitsidwa.

1. Pa zenera lalikulu, dinani NDONDOMEKO YOSUNGA.
2. Kuchokera pazenerali, mutha kusintha kuchuluka kwa magawo, kukula kwa magawo, ndi voliyumu ya buffer kuti mutolere. Pali zosankha zingapo za kukula kwagawo zomwe zilipo kutengera mtundu wa gawo. Dinani -/+ kuti musankhe kuchuluka kwa zosonkhanitsa zanu, kukula kwa zosonkhanitsa, ndi voliyumu ya buffer.
3. Dinani X kuti musunge magawo anu ndikubwerera ku menyu yayikulu.
4. Mukakonzeka kuyambitsa kusonkhanitsa kwanu, dinani START COLLECTION ndikuyambiransoview magawo anu osonkhanitsira.
5. Dinani YES kuti mupitilize kukhazikitsa carousel kapena NO kuti mubwerere ku menyu yayikulu.

Gawo 4C: Kukhazikitsa Carousel
Mutalongosola magawo anu osonkhanitsira, mwakonzeka kukonzekera carousel kuti mutolere.

1. Kwezani machubu osonkhanitsira pa carousel mumayendedwe oyenera.
2. Onetsetsani kuti mwakweza machubu okwanira a protocol yanu yosonkhanitsa.
3. Pang'ono ndi pang'ono ikani carousel pa mbale ya carousel. Onetsetsani kuti bowo lolowera pa carousel likulumikizana ndi pini yokwezeka ya carousel pa mbale ya carousel.
   Onetsetsani kuti mukugwiritsa ntchito machubu olondola osonkhanitsira pamagawo anu. Ngati mwasankha 2.0 mL magawo a magawo a qEV2, ndiye kuti machubu osonkhanitsira 2.0 mL ayenera kugwiritsidwa ntchito. Nthawi zonse lowetsani machubu ofunikira mu carousel ndi zotchingira zoyang'ana mkati. Onetsetsani kuti zivundikiro za machubu sizikukhudzana ndi chophimba cha carousel.

Gawo 4D: Kutsitsa Mzere

1. Chophimba chotsatira chidzakufunsani kuti muyike malo osungiramo ndime. Ngati mukugwiritsa ntchito ndime ya qEVoriginal, qEV1, qEV2, kapena qEV10, kwezani posungira. Ngati mukugwiritsa ntchito imodzi ya qEV, malo osungira sikofunikira.
2. Dinani OK kuti mupitirize.
3. Sewero lotsatira lidzakufunsani ngati mukufuna kutsitsa gawoli. Tikukulimbikitsani kuti muzitsuka mizati yonse ndi buffer yatsopano, yosefedwa ya PBS.
   • Dinani YES ngati mukufuna kutsitsa ndime ndikuwonetsetsa kuti muli ndi buffer yoyenerera pamtundu wa mzati.
   • Dinani NO ngati gawoli lasinthidwa kale. Chophimba chotsatira chidzakufunsani kuti muyambe ndime.
4. Pomwe AFC(V1) ili mumayendedwe osinthira, carousel ikhala pamalo opukutira ndipo chotchingiracho chidzatuluka kuchokera pagawo ndikudutsa pabowo la carousel, kuti aponyedwe kudzera mu chubu cha zinyalala. Dinani Chabwino kuti mupitilize.
5. Dinani Chabwino pamene gawoli lasinthidwa / kusinthidwa ndi voliyumu yovomerezeka ya buffer.
6. Chophimba chotsatira chidzapangitsa wosuta kuti ayike chivundikiro cha carousel pamwamba pa
   AFC (V1). Chophimbacho chimateteza carousel kuti isagwedezeke mosadziwa panthawi yogwira ntchito. Dinani Chabwino kuti mupitilize.

Gawo 4E: Sample Loading and Initiating Collection

1. Chophimba chotsatira chidzakufunsani kuti mukhale ndi buffer yokwanira kuti muyendetse ndimeyi ndikuyika sample. Onetsetsani kuti palibe chotsalira chotsalira pamwamba pa ndime (chotsani chowonjezera chilichonse ndi pipette) musanawonjezere s.ample. Dinani Chabwino kuti mupitilize.
   Ndikofunikira kungonyamula ma sample pa nthawi ino. Kuwonjezera buffer nthawi yomweyo kapena pamaso pa sample walowa kwathunthu mu frit ya ndime zimabweretsa zolakwika sampndi loading.
2. Dinani Chabwino kuti mupitilize. Mzerewu udzayamba kuyenda ndipo carousel idzapita kumalo osungiramo malo. Voliyumu ya bafa iyamba kusonkhanitsidwa pachitsime chapakati cha carousel ndipo kuyatsa kudzasintha kukhala kofiira kuwonetsa kuti ntchitoyo yayamba.
3. Pamene sample yalowa kwathunthu kumtunda kwa gawoli, onjezerani mzati/chosungira ndi kuchuluka koyenera kwa buffer kuti muyambitsenso kusonkhanitsa voliyumu ya buffer.
4. Voliyumu ya buffer ikasonkhanitsidwa, carousel imasunthira pamalo 1, sonkhanitsani voliyumu yomwe yatchulidwa kenako pang'onopang'ono kusuntha gawo lililonse motsatizana. Ngati machubu ang'onoang'ono akufunika kuposa carousel yodzaza kwathunthu, wogwiritsa ntchitoyo adzalangizidwa nthawi yochotsa chivundikiro ndi carousel, m'malo mwa chubu ndi machubu atsopano ndikupitiriza kuthamanga.

Gawo 4F: Kumaliza kuthamanga
Kuthamanga kwatha, ndikofunikira kuyeretsa ndime kuti muwonetsetse kuti palibe transportover pakati pa samples. AFC(V1) idzawongolera wogwiritsa ntchito izi kudzera pamalangizo apakompyuta.

1. Gawo lomaliza litasonkhanitsidwa, kuunikira kowonetsera kudzasanduka kobiriwira ndipo carousel idzabwerera kumalo otayika. Chivundikiro cha carousel ndi machubu otolera tsopano zitha kuchotsedwa bwinobwino.
2. Ngati mugwiritsa ntchito qEVsingle, kukanikiza CHABWINO kudzabwezera wogwiritsa ntchito ku sikirini yakunyumba popeza izi sizingagwiritsidwenso ntchito. Pamitundu ina yonse, wogwiritsa ntchito adzafunsidwa ngati akufuna kuyeretsa gawolo.
3. Sankhani NO kuti mubwererenso pazenera lalikulu ndikuyamba kuthamanga kwina osayeretsa. Izi sizovomerezeka.
4. Sankhani YES kuti muwongoleredwe pakuyeretsa musanabwezedwe pazenera lanyumba.

Gawo 4G: AFC(V1) Tsekani

1. Zimitsani AFC(V1) kumbuyo kwa chidacho.
   Mphunoyo iyenera kutsukidwa bwino ndi 70% ya ethanol solution pambuyo pothamanga komaliza kwa tsiku kuti ateteze kukula kwa mabakiteriya mkati mwa chubu.

KUGWIRA NTCHITO

Gawo 5A: Kukonza ndi Kutumikira kwa chida cha AFC(V1).

• Magawo onse olowa m'malo ayenera kupezeka kudzera ku Izon Science Ltd.
• Lumikizanani ndi nthumwi yanu ya Izon Science kuti mulowe m'malo.
  Tikukulimbikitsani kuti musunge bokosilo ndi zida zoyikapo ngati chida chikufunika kubwezeredwa kuti chigwiritsidwe ntchito.

Zolemba / Zothandizira

IZON AFC(V1) Automated Size Exclusion Chromatography [pdf] Wogwiritsa Ntchito AFC V1, Automated Size Exclusion Chromatography, Size Exclusion Chromatography, Exclusion Chromatography, Chromatography, AFC V1

Zothandizira

• AFC(V1) Buku Logwiritsa Ntchito